School of
Integrative Biology
Steven Burgess

sjb287@illinois.edu

283 Morrill Hall
Office: 217-244-8179

Mail: 505 S. Goodwin, Urbana IL 61801

Steven Burgess
Assistant Professor - Plant Biology

Education

B.Sc. (Hons), 2007, University of Edinburgh, UK
Ph.D., 2011, Imperial College London, UK
Post-doc, 2011-2017, University of Cambridge, UK
Research Fellow, 2018-2021, Institute for Genomic Biology

Sustainable ecosystems using synthetic biology tools to simplify, miniaturize and automate engineering of photosynthetic organisms

Our research aims to provide a sustainable ecosystem for future generations, by working openly and collaboratively with synthetic biology tools to simplify, miniaturize and automate engineering of photosynthetic organisms. Currently, we are focused on developing a mechanistic understanding of (1) adaption of photosynthesis to environmental stress and fluctuations (2) how protein structure influences the activity of genes involved in photosynthesis.

This will allow us to take engineering approaches to introduce improvements in crop performance, as photosynthesis is one of the few remaining traits that breeders have not optimized. To do this we take an interdisciplinary approach, collaborating with researchers on campus to leverage advances in synthetic biology, machine learning, protein engineering, gene editing, genomics and automation to assess the performance of enzyme variants and gene function on photosynthetic performance before translating these findings into crops for field experiments on campus.

The major focus of our lab at the moment is to (1) Develop the smallest know photosynthetic eukaryote, Ostreoccocus tauri, as a model to studying phototrophs, and as a chassis for testing computational predictions of metabolic models and alternate carbon fixation pathways. (2) Developing platforms for directed evolution of photosynthesis enzymes.

Representative Publications

Burgess, S. J., Reyna-Llorens, I., Stevenson, S. R., Singh, P., Jaeger, K., Hibberd, J. M. (2019) Genome-Wide Transcription Factor Binding in Leaves from C3 and C4 Grasses, The Plant Cell, Vol. 31: 2297–2314, DOI: https://doi.org/10.1105/tpc.19.00078

Yu, Z., Boehm, C. R., Hibberd, J. M., Abell, C., Haseloff, J., Burgess, S. J., Reyna-Llorens, I., (2018) Droplet-based microfluidic analysis and screening of single plant cells PLOS ONE https://doi.org/10.1371/journal.pone.0196810

Burgess, S.J., Granero-Moya, I. , Grangé-Guermente, M. J., Boursnell, C., Terry, M.J., Hibberd, J.M. (2016) Ancestral light and chloroplast regulation form the foundations for C4 gene expression Nature Plants 2:16161